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pet28a expression vector  (Addgene inc)


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    Addgene inc pet28a expression vector
    Pet28a Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28a+expression+vector/His-GIV-CTs+(Plasmid+%2369864)/bio_rxiv__64898__2026__03__17__712426-275-10-13
    Average 93 stars, based on 37 article reviews
    pet28a expression vector - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Dynamic Allostery Modulates Catalytic Activity by Modifying the Hydrogen Bonding Network in the Catalytic Site of Human Pin1
    Article Snippet: .. The cDNA encoding human Pin1 PPIase domain was cloned into the pET28a expression vector (Addgene Inc., Cambridge, MA, USA), as reported previously [ , ]. .. Site-directed mutagenesis to yield the S138A mutation in the Pin1 PPIase domain was performed by KOD FX (Toyobo, Osaka, Japan).

    Article Title: Dynamic Allostery Modulates Catalytic Activity by Modifying the Hydrogen Bonding Network in the Catalytic Site of Human Pin1
    Article Snippet: .. The cDNA encoding human Pin1 PPIase domain was cloned into the pET28a expression vector (Addgene Inc., Cambridge, MA, USA), as reported previously [13,30]. .. Site-directed mutagenesis to yield the S138A mutation in the Pin1 PPIase domain was performed by KOD FX (Toyobo, Osaka, Japan).

    Article Title: Botrytis hypersensitive response inducing protein 1 triggers noncanonical PTI to induce plant cell death
    Article Snippet: The hip1 coding sequence devoid of BsaI restriction sites for GreenGate cloning was synthesized by Biocat (Germany). .. For recombinant protein expression, the hip1 sequence was amplified without SP and cloned into the pET28a (+) expression vector (Addgene, USA), eventually having a His-tag at the N-terminus. .. Sequences for cysteine point mutations were synthesized by Biocat (Germany) and cloned into pGreen II for transient expression.

    Article Title: A massively parallel in vivo assay of TdT mutants yields variants with altered nucleotide insertion biases
    Article Snippet: .. The TdT gene was cloned into a pET28a expression vector (Addgene #69864-3) with an N-terminal 10xHis tag, linker, and protease cleavage site. ..

    Article Title: The secreted hypersensitive response inducing protein 1 from Botrytis cinerea displays non-canonical PAMP-activity
    Article Snippet: The Hip1-GFP fusion was constructed by GreenGate cloning , using the following modules: 35S promotor (pGGA004), ER signal sequence (pGGB006), linker-GFP (pGGD001) and RBCS terminator (pGGE001) The Hip1 coding sequence devoid of BsaI restriction sites for GreenGate cloning was synthesized by Biocat (Germany). .. For recombinant protein expression, the Hip1 sequence was amplified without signal peptide and cloned into the pET28a (+) expression vector (Addgene, USA), eventually having a His-tag at the N-terminus. ..

    Expressing:

    Article Title: Dynamic Allostery Modulates Catalytic Activity by Modifying the Hydrogen Bonding Network in the Catalytic Site of Human Pin1
    Article Snippet: .. The cDNA encoding human Pin1 PPIase domain was cloned into the pET28a expression vector (Addgene Inc., Cambridge, MA, USA), as reported previously [ , ]. .. Site-directed mutagenesis to yield the S138A mutation in the Pin1 PPIase domain was performed by KOD FX (Toyobo, Osaka, Japan).

    Article Title: Dynamic Allostery Modulates Catalytic Activity by Modifying the Hydrogen Bonding Network in the Catalytic Site of Human Pin1
    Article Snippet: .. The cDNA encoding human Pin1 PPIase domain was cloned into the pET28a expression vector (Addgene Inc., Cambridge, MA, USA), as reported previously [13,30]. .. Site-directed mutagenesis to yield the S138A mutation in the Pin1 PPIase domain was performed by KOD FX (Toyobo, Osaka, Japan).

    Article Title: Engineering the mode of morphogenetic signal presentation to promote branching from salivary gland spheroids in 3D hydrogels
    Article Snippet: .. The sequence was ligated into the pET28a expression vector (Addgene, Watertown, MA) downstream of the histidine tag, which is downstream of the T7 promoter and is regulatable through an Isopropyl β - D-1-thiogalactopyranoside (IPTG) inducible system. ..

    Article Title: Botrytis hypersensitive response inducing protein 1 triggers noncanonical PTI to induce plant cell death
    Article Snippet: The hip1 coding sequence devoid of BsaI restriction sites for GreenGate cloning was synthesized by Biocat (Germany). .. For recombinant protein expression, the hip1 sequence was amplified without SP and cloned into the pET28a (+) expression vector (Addgene, USA), eventually having a His-tag at the N-terminus. .. Sequences for cysteine point mutations were synthesized by Biocat (Germany) and cloned into pGreen II for transient expression.

    Article Title: A massively parallel in vivo assay of TdT mutants yields variants with altered nucleotide insertion biases
    Article Snippet: .. The TdT gene was cloned into a pET28a expression vector (Addgene #69864-3) with an N-terminal 10xHis tag, linker, and protease cleavage site. ..

    Article Title: Quinone reductase 2 reads H3 serotonylation to support neuronal maturation
    Article Snippet: .. Wildtype and mutant NQO2 inserts were subsequently subcloned into the pET28a expression vector (Addgene #69864) to generate pET28a– NQO2 and pET28a– NQO2 (I129E/I195R), respectively. ..

    Article Title: The secreted hypersensitive response inducing protein 1 from Botrytis cinerea displays non-canonical PAMP-activity
    Article Snippet: The Hip1-GFP fusion was constructed by GreenGate cloning , using the following modules: 35S promotor (pGGA004), ER signal sequence (pGGB006), linker-GFP (pGGD001) and RBCS terminator (pGGE001) The Hip1 coding sequence devoid of BsaI restriction sites for GreenGate cloning was synthesized by Biocat (Germany). .. For recombinant protein expression, the Hip1 sequence was amplified without signal peptide and cloned into the pET28a (+) expression vector (Addgene, USA), eventually having a His-tag at the N-terminus. ..

    Article Title: Immunodominant Mycobacterium tuberculosis Protein Rv1507A Elicits Th1 Response and Modulates Host Macrophage Effector Functions
    Article Snippet: .. Rv1507A was inserted in pET28a expression vector (Addgene, Massachusetts, USA) using Eco RI and Hin dIII restriction sites to construct plasmid pET28a_Rv1507A. .. E.coli BL21(DE3) expression strain was transformed with recombinant constructs and the culture was incubated with 1 mM IPTG for 3 h at 37°C to induce expression of recombinant protein, Rv1507A, which was purified using Ni-NTA affinity column (Qiagen, Hilden, Germany) and eluted with 300 mM imidazole ( ).

    Plasmid Preparation:

    Article Title: Dynamic Allostery Modulates Catalytic Activity by Modifying the Hydrogen Bonding Network in the Catalytic Site of Human Pin1
    Article Snippet: .. The cDNA encoding human Pin1 PPIase domain was cloned into the pET28a expression vector (Addgene Inc., Cambridge, MA, USA), as reported previously [ , ]. .. Site-directed mutagenesis to yield the S138A mutation in the Pin1 PPIase domain was performed by KOD FX (Toyobo, Osaka, Japan).

    Article Title: Dynamic Allostery Modulates Catalytic Activity by Modifying the Hydrogen Bonding Network in the Catalytic Site of Human Pin1
    Article Snippet: .. The cDNA encoding human Pin1 PPIase domain was cloned into the pET28a expression vector (Addgene Inc., Cambridge, MA, USA), as reported previously [13,30]. .. Site-directed mutagenesis to yield the S138A mutation in the Pin1 PPIase domain was performed by KOD FX (Toyobo, Osaka, Japan).

    Article Title: Quinone reductase 2 reads H3 serotonylation to support neuronal maturation
    Article Snippet: .. Wildtype and mutant NQO2 inserts were subsequently subcloned into the pET28a expression vector (Addgene #69864) to generate pET28a– NQO2 and pET28a– NQO2 (I129E/I195R), respectively. ..

    Article Title: Immunodominant Mycobacterium tuberculosis Protein Rv1507A Elicits Th1 Response and Modulates Host Macrophage Effector Functions
    Article Snippet: .. Rv1507A was inserted in pET28a expression vector (Addgene, Massachusetts, USA) using Eco RI and Hin dIII restriction sites to construct plasmid pET28a_Rv1507A. .. E.coli BL21(DE3) expression strain was transformed with recombinant constructs and the culture was incubated with 1 mM IPTG for 3 h at 37°C to induce expression of recombinant protein, Rv1507A, which was purified using Ni-NTA affinity column (Qiagen, Hilden, Germany) and eluted with 300 mM imidazole ( ).

    Sequencing:

    Article Title: Engineering the mode of morphogenetic signal presentation to promote branching from salivary gland spheroids in 3D hydrogels
    Article Snippet: .. The sequence was ligated into the pET28a expression vector (Addgene, Watertown, MA) downstream of the histidine tag, which is downstream of the T7 promoter and is regulatable through an Isopropyl β - D-1-thiogalactopyranoside (IPTG) inducible system. ..

    Article Title: Botrytis hypersensitive response inducing protein 1 triggers noncanonical PTI to induce plant cell death
    Article Snippet: The hip1 coding sequence devoid of BsaI restriction sites for GreenGate cloning was synthesized by Biocat (Germany). .. For recombinant protein expression, the hip1 sequence was amplified without SP and cloned into the pET28a (+) expression vector (Addgene, USA), eventually having a His-tag at the N-terminus. .. Sequences for cysteine point mutations were synthesized by Biocat (Germany) and cloned into pGreen II for transient expression.

    Article Title: The secreted hypersensitive response inducing protein 1 from Botrytis cinerea displays non-canonical PAMP-activity
    Article Snippet: The Hip1-GFP fusion was constructed by GreenGate cloning , using the following modules: 35S promotor (pGGA004), ER signal sequence (pGGB006), linker-GFP (pGGD001) and RBCS terminator (pGGE001) The Hip1 coding sequence devoid of BsaI restriction sites for GreenGate cloning was synthesized by Biocat (Germany). .. For recombinant protein expression, the Hip1 sequence was amplified without signal peptide and cloned into the pET28a (+) expression vector (Addgene, USA), eventually having a His-tag at the N-terminus. ..

    Recombinant:

    Article Title: Botrytis hypersensitive response inducing protein 1 triggers noncanonical PTI to induce plant cell death
    Article Snippet: The hip1 coding sequence devoid of BsaI restriction sites for GreenGate cloning was synthesized by Biocat (Germany). .. For recombinant protein expression, the hip1 sequence was amplified without SP and cloned into the pET28a (+) expression vector (Addgene, USA), eventually having a His-tag at the N-terminus. .. Sequences for cysteine point mutations were synthesized by Biocat (Germany) and cloned into pGreen II for transient expression.

    Article Title: The secreted hypersensitive response inducing protein 1 from Botrytis cinerea displays non-canonical PAMP-activity
    Article Snippet: The Hip1-GFP fusion was constructed by GreenGate cloning , using the following modules: 35S promotor (pGGA004), ER signal sequence (pGGB006), linker-GFP (pGGD001) and RBCS terminator (pGGE001) The Hip1 coding sequence devoid of BsaI restriction sites for GreenGate cloning was synthesized by Biocat (Germany). .. For recombinant protein expression, the Hip1 sequence was amplified without signal peptide and cloned into the pET28a (+) expression vector (Addgene, USA), eventually having a His-tag at the N-terminus. ..

    Amplification:

    Article Title: Botrytis hypersensitive response inducing protein 1 triggers noncanonical PTI to induce plant cell death
    Article Snippet: The hip1 coding sequence devoid of BsaI restriction sites for GreenGate cloning was synthesized by Biocat (Germany). .. For recombinant protein expression, the hip1 sequence was amplified without SP and cloned into the pET28a (+) expression vector (Addgene, USA), eventually having a His-tag at the N-terminus. .. Sequences for cysteine point mutations were synthesized by Biocat (Germany) and cloned into pGreen II for transient expression.

    Article Title: The secreted hypersensitive response inducing protein 1 from Botrytis cinerea displays non-canonical PAMP-activity
    Article Snippet: The Hip1-GFP fusion was constructed by GreenGate cloning , using the following modules: 35S promotor (pGGA004), ER signal sequence (pGGB006), linker-GFP (pGGD001) and RBCS terminator (pGGE001) The Hip1 coding sequence devoid of BsaI restriction sites for GreenGate cloning was synthesized by Biocat (Germany). .. For recombinant protein expression, the Hip1 sequence was amplified without signal peptide and cloned into the pET28a (+) expression vector (Addgene, USA), eventually having a His-tag at the N-terminus. ..

    Mutagenesis:

    Article Title: Quinone reductase 2 reads H3 serotonylation to support neuronal maturation
    Article Snippet: .. Wildtype and mutant NQO2 inserts were subsequently subcloned into the pET28a expression vector (Addgene #69864) to generate pET28a– NQO2 and pET28a– NQO2 (I129E/I195R), respectively. ..

    Construct:

    Article Title: Immunodominant Mycobacterium tuberculosis Protein Rv1507A Elicits Th1 Response and Modulates Host Macrophage Effector Functions
    Article Snippet: .. Rv1507A was inserted in pET28a expression vector (Addgene, Massachusetts, USA) using Eco RI and Hin dIII restriction sites to construct plasmid pET28a_Rv1507A. .. E.coli BL21(DE3) expression strain was transformed with recombinant constructs and the culture was incubated with 1 mM IPTG for 3 h at 37°C to induce expression of recombinant protein, Rv1507A, which was purified using Ni-NTA affinity column (Qiagen, Hilden, Germany) and eluted with 300 mM imidazole ( ).



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